Minimal SO(10) Supersymmetric Grand Unified Theories (SUSY GUTs) are one of interesting candidates for physics beyond the standard model: all quarks and leptons (including right-handed neutrino) in one generation are unified into 16-dimensional representation of SO(10), and two Higgs doublets in SUSY standard model are also unified in 10-dimensional representation. In this talk, third family Yukawa unification, as predicted by minimal SO(10) GUTs, is revisited. Since GUT-scale Yukawa couplings are very sensitive to SUSY threshold corrections at weak scale, Yukawa unification hypothesis strongly constrains low-energy SUSY particle spectrum. I show constraints on the SUSY spectrum requiring Yukawa unification, and then discuss possible SUSY breaking scenarios in order to achieve Yukawa unification.