Special Colloquium, March 18, 2004

Three-Dimensional Diffraction Microscopy and Its Applications in Structural Biology

Jianwei Miao

Stanford Linear Accelerator Center
Stanford University

When a coherent diffraction pattern is sampled at a spacing sufficiently finer than the Bragg peak frequency (i.e. the inverse of the sample size), the phase information is in principle embedded inside the diffraction pattern, and can be directly retrieved by using an iterative process. In combination of this oversampling phasing method with either coherent X-rays or electrons, a novel form of diffraction microscopy has recently been developed to image nano-crystals, non-crystalline structures and biological samples. In this talk, I will present the principle of the oversampling method, discuss the first experimental demonstration of this microscope, and illustrate some applications in structural biology.

11:30 a.m., Smith Laboratory, Room 1094

Refreshments served in Smith 1094 at 11:00 a.m.